Human liposarcoma cell line, SW872, secretes cholesteryl ester transfer protein in response to cholesterol

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Human liposarcoma cell line, SW872, secretes cholesteryl ester transfer protein in response to cholesterol.

Cholesteryl ester transfer protein (CETP) mediates the exchange of phospholipids and neutral lipids between the plasma lipoproteins, and plays an important role in high density lipoprotein (HDL) metabolism. While there are reports of low-level CETP secretion from cultured cells, the lack of a good model cell line has hampered the detailed study of CETP regulation and secretion. In this study, w...

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Overexpression of full-length cholesteryl ester transfer protein in SW872 cells reduces lipid accumulation.

Cells produce two cholesteryl ester transfer protein (CETP) isoforms, full-length and a shorter variant produced by alternative splicing. Blocking synthesis of both isoforms disrupts lipid metabolism and storage. To further define the role of CETP in cellular lipid metabolism, we stably overexpressed full-length CETP in SW872 cells. These CETP(+) cells had several-fold higher intracellular CETP...

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Adipose tissue cholesteryl ester transfer protein mRNA in response to probucol treatment: cholesterol and species dependence.

Probucol treatment results in an increase in plasma concentrations of cholesteryl ester transfer protein (CETP) which may account, in part, for the effects of this agent on plasma concentrations of HDL cholesterol. We have examined the mechanism by which probucol increases plasma CETP and have determined the associated changes in the plasma distribution of high density lipoprotein (HDL) particl...

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1996

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)42025-5